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HPLC with chemiluminescence detection of serum levels of precolumn derivatized fluoropyrimidine compounds
Summary
Type:
HPLC
Application #:
107101
Catalog#:
720014.40 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4x250mm
Detection:
chemiluminescence
Conditions:
eluent: methanol - 4mM phosphate buffer pH 7.5 (50:50, v/v)
flow rate 0.6ml/min
Authors:
Yoshida Sh
Source:
J Chromatogr 530 (1990) 57 - 64
Details
Legend: Fig. 3 HPLC profile of A) pyrimidine standard mixture, B) serum extract, derivatized with 7-(diethylamino)-3-[4-((iodoacetyl)amino)phenyl]-4-methylcoumarin in acetone in the presence of 18-crown-6
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